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human pooled metabolic crispr ko library  (Addgene inc)


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    Addgene inc human pooled metabolic crispr ko library
    Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence . (A – B) Schematic representation of RNA-Seq (A) and <t>CRISPR</t> <t>KO</t> screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change ≥2, FDR ≤0.1; Cutoff for CRISPR screen: pval ≤0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C) . (E) ACSS2 protein expression was determined by western blotting in the indicated cells. β-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.
    Human Pooled Metabolic Crispr Ko Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pooled+metabolic+crispr+ko+library/pmc11462069-46-0-6?v=Addgene+inc
    Average 93 stars, based on 22 article reviews
    human pooled metabolic crispr ko library - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Acetate drives ovarian cancer quiescence via ACSS2-mediated acetyl-CoA production"

    Article Title: Acetate drives ovarian cancer quiescence via ACSS2-mediated acetyl-CoA production

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2024.102031

    Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence . (A – B) Schematic representation of RNA-Seq (A) and CRISPR KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change ≥2, FDR ≤0.1; Cutoff for CRISPR screen: pval ≤0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C) . (E) ACSS2 protein expression was determined by western blotting in the indicated cells. β-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.
    Figure Legend Snippet: Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence . (A – B) Schematic representation of RNA-Seq (A) and CRISPR KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change ≥2, FDR ≤0.1; Cutoff for CRISPR screen: pval ≤0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C) . (E) ACSS2 protein expression was determined by western blotting in the indicated cells. β-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.

    Techniques Used: Biomarker Discovery, RNA Sequencing, CRISPR, Comparison, Quantitative Proteomics, Expressing, Western Blot, Control



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    human pooled metabolic crispr ko library  (Addgene inc)
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    Addgene inc human pooled metabolic crispr ko library
    Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence . (A – B) Schematic representation of RNA-Seq (A) and <t>CRISPR</t> <t>KO</t> screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change ≥2, FDR ≤0.1; Cutoff for CRISPR screen: pval ≤0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C) . (E) ACSS2 protein expression was determined by western blotting in the indicated cells. β-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.
    Human Pooled Metabolic Crispr Ko Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pooled+metabolic+crispr+ko+library/pmc11462069-46-0-6?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    human pooled metabolic crispr ko library - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier
    lentiviral production human pooled metabolic crispr ko library  (Addgene inc)
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    Addgene inc lentiviral production human pooled metabolic crispr ko library
    Figure 1: Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence. (AeB) Schematic representation of RNA-Seq (A) and <t>CRISPR</t> KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change 2, FDR 0.1; Cutoff for CRISPR screen: pval 0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C). (E) ACSS2 protein expression was determined by western blotting in the indicated cells. b-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.
    Lentiviral Production Human Pooled Metabolic Crispr Ko Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pooled+metabolic+crispr+ko+library/pm39304063-72-2-10?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    lentiviral production human pooled metabolic crispr ko library - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence . (A – B) Schematic representation of RNA-Seq (A) and CRISPR KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change ≥2, FDR ≤0.1; Cutoff for CRISPR screen: pval ≤0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C) . (E) ACSS2 protein expression was determined by western blotting in the indicated cells. β-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.

    Journal: Molecular Metabolism

    Article Title: Acetate drives ovarian cancer quiescence via ACSS2-mediated acetyl-CoA production

    doi: 10.1016/j.molmet.2024.102031

    Figure Lengend Snippet: Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence . (A – B) Schematic representation of RNA-Seq (A) and CRISPR KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change ≥2, FDR ≤0.1; Cutoff for CRISPR screen: pval ≤0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C) . (E) ACSS2 protein expression was determined by western blotting in the indicated cells. β-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.

    Article Snippet: Human pooled metabolic CRISPR KO library (Addgene #110066), pLV-mCherry-hCdt1(1–100)ΔCy (Addgene #193759), and pCDH-EF1-mVenus-p27K − (Addgene #176651) were obtained from Addgene.

    Techniques: Biomarker Discovery, RNA Sequencing, CRISPR, Comparison, Quantitative Proteomics, Expressing, Western Blot, Control

    Figure 1: Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence. (AeB) Schematic representation of RNA-Seq (A) and CRISPR KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change 2, FDR 0.1; Cutoff for CRISPR screen: pval 0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C). (E) ACSS2 protein expression was determined by western blotting in the indicated cells. b-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.

    Journal: Molecular metabolism

    Article Title: Acetate drives ovarian cancer quiescence via ACSS2-mediated acetyl-CoA production.

    doi: 10.1016/j.molmet.2024.102031

    Figure Lengend Snippet: Figure 1: Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence. (AeB) Schematic representation of RNA-Seq (A) and CRISPR KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change 2, FDR 0.1; Cutoff for CRISPR screen: pval 0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C). (E) ACSS2 protein expression was determined by western blotting in the indicated cells. b-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.

    Article Snippet: Plasmids and lentiviral production Human pooled metabolic CRISPR KO library (Addgene #110066), pLVmCherry-hCdt1(1e100)DCy (Addgene #193759), and pCDH-EF1mVenus-p27K (Addgene #176651) were obtained from Addgene.

    Techniques: Biomarker Discovery, RNA Sequencing, CRISPR, Comparison, Quantitative Proteomics, Expressing, Western Blot, Control